Green mouse lines

Characteristics of green mice that we are distributing. At first we distributed the green mice with B6C3F1 back ground. However, we are now distributing the green mice produced from C57BL/6 mouse eggs.

C57BL/6-Tg(CAG-EGFP)C15-001-FJ001Osb : practically homo lethal. very bright in hemizygous.
The chromose integration site is D region of chromosome 15.
This line is distributed through Jackson Lab as (C57BL/6-TgN(ACTbEGFP)1Osb Jackson Labs stock 003291)

(old name ; TgN(b-act-EGFP)Osb, C57BL/6 Tg15(act-EGFP)Osb1,
C57BL/6 Cr Slc TgN(act-EGFP)OsbC15-001-FJ001, C57BL/6-Tg(Act-EGFP)C15-001-FJ001Osb)
C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb : produce healthy homozygous. homozygous is as bright as TG15, hemizygous is less bright than homozygous. (Chromosome 14D1)
This line is distributed through RIKEN, CARD and NIBIO .
Alternatively, you may obtain the mice from a Japanese breeder, Japan SLC.

(old name ; C57BL/6 TgN(act-EGFP)OsbY01, C57BL/6 Tg14(act-EGFP)OsbY01,
C57BL/6 Cr Slc TgN(act-EGFP)OsbC14-Y01-FM131, C57BL/6-Tg(ACT-EGFP)C14-Y01-FM131Osb)
FACS spleen FACS thymus mouse transfer

Transgenes integration site in 142 transgenic mice (Genomics, 2002, 80(6):564-74)
line no.	transgenes	chromosomal locus	status
9	CX-EGFP + XPG	Chr 9 D	-
10	CX-EGFP + XPG	Chr 18 C	-
11	CX-EGFP + XPG	Chr 12 B3 - C1 proximal	-
14	CX-EGFP + XPG	Chr 2 B proximal	cryopreserved
16	CX-EGFP + XPG	Chr 6 C2 – C3 proximal	cryopreserved
17	CX-EGFP + XPG	Chr 4 A2 - A3	cryopreserved
18	CX-EGFP + XPG	Chr 5 G2	cryopreserved
32	CX-EGFP	Chr 13 C2	cryopreserved
33	CX-EGFP	Chr 10 D1 – D2 proximal	-
35	CX-EGFP	Chr 3 A3 proximal	cryopreserved
37	CX-EGFP	Chr 10 C2 - C3	cryopreserved
38^a	CX-EGFP	Chr X D + Chr 16C3.1 - C3.2	cryopreserved for Chr X-linked transgene
39^c	CX-EGFP	Chr 11 B1.1 + t (2 ; 10) (H1 - H2 ; A2)	-
42	CX-EGFP	Chr 15 D3	cryopreserved
43	CX-EGFP	Chr 3 A2 - A3	-
44	CX-EGFP	Chr 14 E2.1 distal – E2.2	-
45	CX-EGFP	Chr 1 C1.3 - C2	cryopreserved
46	CX-EGFP	Chr 5 A3 - B1	-
47^a	CX-EGFP	Chr 17 E2 distal – E3 + Chr 1 F distal - G1	cryopreserved
48	CX-EGFP	Chr 11 A3.2 - A4 proximal	-
49	CX-EGFP	Chr 14 C - D1 distal	-
50	CX-EGFP	Chr X C3	cryopreserved
51	CX-EGFP	Chr 10 C2 - C3	-
52	CX-EGFP	Chr 2 D - E1	cryopreserved
53^b	CX-EGFP	Chr Y distal end, Chr 5 G2 distal	cryopreserved for Chr 5 transgene
54	CX-EGFP	Chr 13 D1 distal - D2.1	cryopreserved
55^a	CX-EGFP	Chr 12 C2 - C3 proximal + Chr 5 G	cryopreserved
56	CX-EGFP	Chr 5 C1 distal - C3 proximal	cryopreserved
57	CX-EGFP	Chr 17 D	cryopreserved
58^c	CX-EGFP	t (11;14)(A5;E3)	cryopreserved
59	CX-EGFP	Chr 10 D1 - D2 proximal	cryopreserved
60	CX-EGFP	Chr 8 B2 - B3.1	cryopreserved
61	CX-EGFP	Chr 4 B3 distal – C1	cryopreserved
62	CX-EGFP	Chr 4 B3 - C1	cryopreserved
64	CX-EGFP	Chr 1 C4 - C5	cryopreserved
65	CX-EGFP	Chr 4 C6 - C7 proximal	cryopreserved
66^b	CX-EGFP	Chr 15 B3.2 - C, Chr 19 D2	cryopreserved
68	CX-EGFP	Chr 17 E2 - E3	cryopreserved
69	CX-EGFP	Chr 8 C3 - C5	cryopreserved
70	CX-EGFP	Chr 8 B1.3 - B2	cryopreserved
71	CX-EGFP	Chr 16 B5	cryopreserved
72	CX-EGFP	Chr 9 E3.3 distal - F1 proximal	cryopreserved
73	CX-EGFP	Chr 13 A4 - A5 proximal	cryopreserved
74	CX-EGFP	Chr 6 C1	cryopreserved
75	CX-EGFP	Chr 17 E2	cryopreserved
76	CX-EGFP	Chr X D distal	-
77	CX-EGFP	Chr 3 G3 distal - H1	cryopreserved
85	CX-EGFP	Chr 18 B2 – B3 proximal	-
86	CX-EGFP	Chr 9 D	cryopreserved
87^a	CX-EGFP	Chr 12 A1.1distal + Chr 12 E	-
88	CX-EGFP	Chr 6 A3.1 – A3.2	cryopreserved
89	CX-EGFP	Chr 7 B5 distal - C	cryopreserved
90	CX-EGFP	Chr 2 F1	cryopreserved
91	CX-EGFP	Chr 8 B1.1 - B1.3	cryopreserved
93^a	CX-EGFP	Chr 5 B3 + Chr 8 B3.1 - B3.2	-
94	CX-EGFP	Chr 19 D1 - D2	cryopreserved
116^a	CX-EGFP	Chr 2 D + Chr 18 D3 - E1	cryopreserved
117	CX-EGFP	Chr 4 C3	-
118	CX-EGFP	Chr 4 C1 distal - C3 proximal	-
119	CX-EGFP	Chr 7 E3 - F1	cryopreserved
120	CX-EGFP	Chr 2 A3 proximal	cryopreserved
121	CX-EGFP	Chr 12 F1	-
122	CX-EGFP	Chr 12 C2	-
123^c	CX-EGFP	ins (5 ; 2) (G2 ; E2 - F1)	-
124	CX-EGFP	Chr 6 C2	cryopreserved
125	CX-EGFP	Chr 9 E3.3	-
126	CX-EGFP	Chr 5 E1 distal - E2 proximal	cryopreserved
127	CX-EGFP	Chr 10 B2	cryopreserved
128^c	CX-EGFP	t (3 ; 19) (F1 ; A)	-
129	CX-EGFP	Chr 2 C3 distal – D proximal	-
130	CX-EGFP	Chr 2 E1 distal - E3	cryopreserved
131	CX-EGFP	Chr 14 D1	cryopreserved
132	CX-EGFP＋acr3-EGFP	Chr 3 A3	cryopreserved
133	CX-EGFP＋acr3-EGFP	Chr 7 B5 - C	cryopreserved
134	CX-EGFP＋acr3-EGFP	Chr 1 A2 - A3	cryopreserved
135	CX-EGFP＋acr3-EGFP	Chr 3 C - D	cryopreserved
136	CX-EGFP＋acr3-EGFP	Chr 9 F1	cryopreserved
137	CX-EGFP＋acr3-EGFP	Chr 5 C3.2 - C3.3	cryopreserved
138	CX-EGFP＋acr3-EGFP	Chr 1 D	cryopreserved
139	CX-EGFP	Chr X A3	cryopreserved
141	CX-EGFP + XPG	Chr 1 C3	cryopreserved
142	CX-EGFP + XPG	Chr 15 A1	-
143	CX-EGFP + XPG	Chr 8 C5	cryopreserved
160	CX-EGFP	Chr 17 E2	cryopreserved
161	CX-EGFP	Chr 10 C1 proximal	cryopreserved
162^a	CX-EGFP	Chr 6 E3 distal - F1 + Chr 6 D3 distal	-
165	CX-EGFP	Chr 9 F2 - F3 proximal	cryopreserved
170	CX-EGFP	Chr 5 C3.1 - C3.3	cryopreserved
201	CX-EGFP-lox71	Chr 17 C	cryopreserved
202	CX-EGFP-lox71	Chr 12 C1 distal - C3 proximal	cryopreserved
203	CX-EGFP-lox71	Chr 2 C1.3 - C2	cryopreserved
204	CX-EGFP-lox71	Chr 11 A3.2 - A4	cryopreserved
206	CX-EGFP-lox71	Chr 5 A3	-
207	CX-EGFP-lox71	Chr 9 E3.3 - E4	-
208^c	CX-EGFP-lox71	Chr 9 E3.2 - E3.3, t (3 ; 14) (B1 ; E2.2-E2.3)	cryopreserved
210	CX-EGFP-lox71	Chr 12 E	cryopreserved
211	CX-EGFP-lox71	Chr 14 E2.1	cryopreserved
212^a	CX-EGFP-lox71	Chr 7C + Chr 9 E3.1 distal - E3.3 proximal	cryopreserved
213^b	CX-EGFP-lox71	Chr 2 E1 distal - E2, Chr 6 D3 distal - E2 + Chr 13 D1 distal - D2.1	-
215	CX-EGFP-lox71	Chr X F2	cryopreserved
217	CX-EGFP-lox71	Chr 15 B3.2 - B3.3	cryopreserved
218	CX-EGFP-lox71	Chr 2 A3 - B proximal	cryopreserved
219	CX-EGFP-lox71	Chr 10 D1 - D2 proximal	cryopreserved
220	CX-EGFP-lox71	Chr 7 F3 distal - F4	-
221^a	CX-EGFP-lox71	Chr 6 D1 distal - D3 proximal + Chr 16 B1 - B2	-
222	CX-EGFP-lox71	Chr 10 C3-D1	cryopreserved
223	CX-EGFP-lox71	Chr X F4	cryopreserved
224^c	CX-EGFP-lox71	t (4 ; 15) (C6 - C7 ; A1 distal)	-
225^a	CX-EGFP-lox71	Chr 14 A3 distal - B + Chr 14 D1	cryopreserved
226^a	CX-EGFP-lox71	Chr 4 C3 distal - C4 + Chr 9 E3.3 - F1 proximal	cryopreserved
227	CX-EGFP-lox71	Chr 8 B1.3 - B3.2	-
228^a	CX-EGFP-lox71	Chr 2 B + Chr 3 B distal - C	cryopreserved
229	CX-EGFP-lox71	Chr 1 H1 - H2.1	cryopreserved
230^a	CX-EGFP-lox71	Chr 5 G1.1 - G1.3 + Chr 6 C1 distal - C2	cryopreserved
231	CX-EGFP-lox71	Chr 1 E2.1 distal - E2.3 proximal	cryopreserved
233	CX-EGFP-lox71	Chr 18 B2 - B3	cryopreserved
234	CX-EGFP-lox71	Chr 5 G1.1 - G1.3	cryopreserved
235	CX-EGFP-lox71	Chr 2 C1.1 distal - C1.3	-
236^c	CX-EGFP-lox71	t (3 ; 13) (B ; D1)	-
237	CX-EGFP-lox71	Chr 4 B3 - C1	cryopreserved
238	CX-EGFP-lox71	Chr 8 B1.3 - B3.1	cryopreserved
240	CX-EGFP-lox71	Chr 1 A5	cryopreserved
241	CX-EGFP-lox71	Chr 9 A3 - A4 proximal	cryopreserved
242^a	CX-EGFP-lox71	Chr 3 G1.1 distal - G3 proximal + Chr 13 B3 - C1.1	cryopreserved
243	CX-EGFP-lox71	Chr 11 A3.1 - A3.3	cryopreserved
244	CX-EGFP-lox71	Chr 2 G1 distal - G3 proximal	cryopreserved
245	CX-EGFP-lox71	Chr 6 A3.2 - A3.3	cryopreserved
247^a	CX-EGFP-lox71	Chr 6 D2 - D3 + Chr 10 B2 - B3	cryopreserved
248	CX-EGFP-lox71	Chr 12 D3 distal - E proximal	cryopreserved
249	CX-EGFP-lox71	Chr 3 H3 - H4	cryopreserved
250	CX-EGFP-lox71	Chr 2 B distal	cryopreserved
251	CX-EGFP-lox71	Chr 2 A3	cryopreserved
252	CX-EGFP-lox71	Chr 2 C3	-
253	CX-EGFP-lox71	Chr 12 C2 distal – C3 proximal	cryopreserved
254	CX-EGFP-lox71	Chr 15 E2	cryopreserved
255	CX-EGFP-lox71	Chr 16 C1.1	cryopreserved
256	CX-EGFP-lox71	Chr 2 B	-
257^d	CXN-EGFP	Chr 12 D3
258^d	CXN-EGFP	Chr 5 F distal
259^d	CXN-EGFP	Chr 2 B distal
260^d	CXN-EGFP	Chr 2 D
261^d	CXN-EGFP	Chr 11 B1.3

^aTransgenes were integrated into two chromosome sites.
^bTwo types of cells, in which the chromosome locations of the transgenes were different, were observed (mosaic condition).
^cRearranged chromosomes were detected.
^dES cell lines were transmitted to their germlilne.
Transgenic mouse lines #9 - #165 and #170 - #256 excluding #130 - #132 (C57BL/6 strain) were made from B6C3F1 and B6D2F1 mouse strain, respectively.
#257 - #258 and #259 - #261 were transgenic mice made by injecting R1 and D3 ES cell lines, respectively, to blastocysts derived from C57BL/6 strains.