PCR amplification of EGFP gene
Pr385 5'-tgaaccgcat cgagctgaag gg-3'
Pr386 5'-tccagcagga ccatgtgatc gc-3'
water
9.2
DNA
1.0
10xbuffer
1.5 (without MgCl2)
2.5mM dNTP
1.2 (final 0.2mM)
25mM MgCl2
0.9 (final 1.5mM)
Pr385
0.3 (final 1ƒÊM)
Pr386
0.3 (final 1ƒÊM)
Takara ExTaq
0.3
total
15.0
94 C for 30 seconds
65 C for 30 seconds
72 C for 30 seconds
‚˜35-40 cycles
You will be able to see a band in approximately 307bp area